Gas chromatography (GC) and liquid chromatography (LC) are two commonly used chromatographic analysis techniques, which have significant differences in many aspects, mainly reflected in sample properties, mobile phase, stationary phase, analytical object, and application fields.
1. Sample properties:
Gas chromatographs are mainly suitable for analyzing volatile and thermally stable samples, such as gas, volatile liquid or solid samples that have been processed and converted into gas.
Liquid chromatographs are suitable for analyzing samples with high boiling points, poor thermal stability, and low volatility, such as biological samples and compounds in organic solvents.
2. Mobile phase:
The mobile phase of a gas chromatograph is gas, usually inert gas (such as nitrogen, helium) or carrier gas (such as hydrogen), which flows at a certain flow rate in the chromatographic column, driving the separation of sample components in the column.
The mobile phase of a liquid chromatograph is a liquid, usually water, organic solvent, or mixed solvent. The mobile phase is pumped into the chromatographic column by a high-pressure pump, and the sample components are distributed and separated between the mobile phase and the stationary phase.
3. Fixed phase:
The stationary phase of a gas chromatograph is usually a solid adsorbent or polymer, which interacts with the sample components through physical adsorption or chemical bonding to achieve separation.
The stationary phase of a liquid chromatograph is a solid adsorbent (such as silica gel, alumina, etc.) or a polymer (such as polystyrene, polymethyl methacrylate, etc.), which separates from the sample components through van der Waals forces, hydrogen bonding, ion exchange, and other interactions.
4. Analysis object:
Gas chromatographs are mainly used for analyzing gas samples, volatile organic solvents and compounds, as well as complex samples that have been derivatized.
Liquid chromatography is more suitable for analyzing organic compounds with high boiling points, poor thermal stability, and low volatility, such as biological macromolecules (proteins, nucleic acids, etc.), polar compounds, ionic compounds, etc.
5. Application field:
Gas chromatographs are widely used in fields such as petrochemicals, environmental monitoring, food safety, and drug analysis, especially in the analysis of gases and volatile organic compounds, which have unique advantages.
Liquid chromatography plays an important role in the fields of biological science, medicine, environmental science, food science, etc., especially in the analysis of biomolecules, polar compounds, and ionic compounds, with wide applications.
In addition, there are certain differences between gas chromatographs and liquid chromatographs in terms of detectors, injection methods, chromatographic columns, etc. These differences give the two technologies different advantages and applicability in different fields.
ZC-215 Insulating Oil Gas Chromatography Analyzer is a technique for separating and analyzing multi-component mixtures. It mainly utilizes the differences in boiling points, polarity, and adsorption coefficients of each component in the sample in the chromatographic column to separate each component in the column, and conducts qualitative and quantitative analysis of the separated components.
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